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In this project we use an experimental approach to investigate two highly debated questions in ecology. What are the contributions of deterministic versus stochastic forces to variation in community composition and functioning? And how does dispersal alter these contributions? We performed a field experiment focusing on plant litter microbial communities. Plant litter decomposition influences nutrient cycling in terrestrial ecosystems, including the balance of plant carbon (C) released in the atmosphere as CO 2 versus stored in the soil. By minimizing differences in initial community composition, minimizing environmental variation in the experiment, and taking replicate measurements at multiple levels, we quantified the contribution of stochastic effects across multiple levels of biodiversity in a natural environment. In this plant litter system, stochastic processes contributed to a large degree of variation in taxonomic composition at both a community and population level. However, these stochastic effects also altered ecosystem functioning. Varying bacterial dispersal rates significantly impacted both community composition and functioning. We further highlight measurement error as a missing factor in many studies attempting to disentangle the relative contribution of deterministic and stochastic processes. Measurement error may differ depending on the context of a study (environment, organisms etc.) and technical factors, and represents an important component of observed variation to be considered. Ultimately, the ability to accurately quantify stochastic processes is paramount to determining the predictability of ecosystem structure and functioning, whether focused on bacteria that degrade plant litter, microbes in the human gut, or patterns of global biodiversity.
Last Modified: 08/24/2017 Modified by: Jennifer B Martiny
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Published on September 21, 2022 by Tegan George . Revised on July 18, 2023.
When starting your thesis or dissertation process, one of the first requirements is a research proposal or a prospectus. It describes what or who you want to examine, delving into why, when, where, and how you will do so, stemming from your research question and a relevant topic .
The proposal or prospectus stage is crucial for the development of your research. It helps you choose a type of research to pursue, as well as whether to pursue qualitative or quantitative methods and what your research design will look like.
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What should your proposal contain, dissertation question examples, what should your proposal look like, dissertation prospectus examples, other interesting articles, frequently asked questions about proposals.
Prior to jumping into the research for your thesis or dissertation, you first need to develop your research proposal and have it approved by your supervisor. It should outline all of the decisions you have taken about your project, from your dissertation topic to your hypotheses and research objectives .
Depending on your department’s requirements, there may be a defense component involved, where you present your research plan in prospectus format to your committee for their approval.
Your proposal should answer the following questions:
Ultimately, your proposal should persuade your supervisor or committee that your proposed project is worth pursuing.
Strong research kicks off with a solid research question , and dissertations are no exception to this.
Dissertation research questions should be:
An easy rule of thumb is that your proposal will usually resemble a (much) shorter version of your thesis or dissertation. While of course it won’t include the results section , discussion section , or conclusion , it serves as a “mini” version or roadmap for what you eventually seek to write.
Be sure to include:
The length of your proposal varies quite a bit depending on your discipline and type of work you’re conducting. While a thesis proposal is often only 3-7 pages long, a prospectus for your dissertation is usually much longer, with more detailed analysis. Dissertation proposals can be up to 25-30 pages in length.
Writing a proposal or prospectus can be a challenge, but we’ve compiled some examples for you to get your started.
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The research methods you use depend on the type of data you need to answer your research question .
A thesis or dissertation outline is one of the most critical first steps in your writing process. It helps you to lay out and organize your ideas and can provide you with a roadmap for deciding what kind of research you’d like to undertake.
Generally, an outline contains information on the different sections included in your thesis or dissertation , such as:
A well-planned research design helps ensure that your methods match your research aims, that you collect high-quality data, and that you use the right kind of analysis to answer your questions, utilizing credible sources . This allows you to draw valid , trustworthy conclusions.
The priorities of a research design can vary depending on the field, but you usually have to specify:
A dissertation prospectus or proposal describes what or who you plan to research for your dissertation. It delves into why, when, where, and how you will do your research, as well as helps you choose a type of research to pursue. You should also determine whether you plan to pursue qualitative or quantitative methods and what your research design will look like.
It should outline all of the decisions you have taken about your project, from your dissertation topic to your hypotheses and research objectives , ready to be approved by your supervisor or committee.
Note that some departments require a defense component, where you present your prospectus to your committee orally.
Formulating a main research question can be a difficult task. Overall, your question should contribute to solving the problem that you have defined in your problem statement .
However, it should also fulfill criteria in three main areas:
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Phd candidate alexandra peña hernández successfully defended her thesis.
Dept of Plant Soil and Microbial Sciences - August 25, 2024
“Integrated strategies for improved management of cercospora leaf spot on sugarbeet,” working with lead advisor Jaime Wilbur and the MSU Sugarbeet and Potato Research Unit.
PhD candidate Alexandra Peña Hernández successfully defended her thesis: “ Integrated strategies for improved management of Cercospora leaf spot on sugarbeet,” working with lead advisor Jaime Wilbur and the MSU Sugarbeet and Potato Research Unit .
Faculty, students and staff packed the room while Alex described her process of science and discovery—including many hours driving, working in the field, and a low-key flame thrower , which was attached to a tractor and drove over the crops to test the effects of extreme heat on sugarbeet leaf spot overwintering. “This project was a lot of work, Alex says, “but I have a great team and we all help each other.”
Alex came to PSM after getting her bachelor’s degree in biosystem and agricultural engineering in the College of Engineering here at MSU. “I had met Jaime during a senior design research experience and did an internship with an agronomy company” Alex said. “And that really helped me decide to go to graduate school.” Alex is Jaime Wilbur’s first PhD student to graduate since starting with PSM in 2018.
Alex, who started with an eye on a master’s degree in fall 2019, quickly made the transition to the PhD program. Alex’s plans include finishing up her project, getting married in October, and eventually working in industry or with the USDA. “It’s been such an amazing experience to work with this team,” Alex said.
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2024 Theses Doctoral
Fang, Yimeng
Epigenetics involves heritable changes in an individual’s traits resulting from variations in gene expression without alterations to the DNA sequence. In eukaryotes, genomic DNA is usually folded with histones into chromatin. Post-translational modifications (PTMs) on histones not only play crucial roles in regulating various biological processes, including gene expression, but also store the majority of epigenetic information. A fundamental question in this field is how cells transmit these PTMs to their progeny. Before I began my thesis research, a well-established dogma in the field was that parental histones containing PTMs are symmetrically distributed to daughter DNA strands during DNA replication. These modified histones serve as templates for PTM duplication, thereby restoring the original chromatin states on both daughter strands. Several histone chaperones have been identified as regulators of parental histone segregation. However, their impact on epigenetic inheritance is controversial, which I reasoned is due to the lack of proper systems to examine epigenetic inheritance. This prompted me to use the unique characteristics of fission yeast heterochromatin as a model of epigenetic inheritance. In this organism, heterochromatin formation involves two distinct steps: establishment and inheritance. Reporter systems have been established to allow precise examination of heterochromatin inheritance. However, parental histone segregation pathways have not been characterized in this organism, and their impact on heterochromatin inheritance is unknown. My thesis work investigates the role of parental histone chaperones in regulating parental histone segregation and epigenetic inheritance in fission yeast. It comprises 5 chapters: Chapter 1 introduces epigenetics, with a focus on chromatin-based epigenetic inheritance. It also highlights the unique features of fission yeast heterochromatin that make it an excellent model for studying epigenetic inheritance.Chapter 2 is the focus of my thesis work. I employed inheritance-specific reporters in fission yeast to investigate the roles of three parental histone chaperones on epigenetic inheritance. In addition, in collaboration with Dr. Zhiguo Zhang’s lab, I adapted the Enrichment and Sequencing of Protein-Associated Nascent DNA (eSPAN) method, a recently developed technique designed to quantify the bias of specific proteins at replication forks, to examine parental histone segregation in fission yeast. My analyses demonstrated a critical role for parental histone segregation in epigenetic inheritance. Moreover, I discovered that both the symmetric segregation of parental histones and their density on daughter strands are critical for this process. Chapter 3 uncovers a novel function of a DNA replication protein Mrc1 in regulating epigenetic inheritance, distinct from its established roles in DNA replication checkpoint activation and replication speed control. I demonstrated the critical role of Mrc1 in regulating the symmetrical transfer of parental histone and the proper inheritance of heterochromatin. These results provide essential mechanistic insights into the function of Mrc1. Chapter 4 explores the function of an additional DNA replication protein and histone chaperone, Swi7 (Pol alpha). I have found that mutations in Swi7 lead to defects in parental histone segregation and heterochromatin inheritance, laying a strong foundation to further investigate its mechanism of action. Chapter 5 discusses potential future research directions that can build upon my thesis work.In conclusion, my thesis represents a thorough examination of parental histone chaperones in regulating epigenetic inheritance in fission yeast. By combining innovative genetic assays and advanced methodologies such as eSPAN, I have provided critical insights into the molecular mechanisms of epigenetic inheritance. In addition, the assays that I have developed during my thesis work also pave the way for future studies aimed at elucidating the mechanism of epigenetic inheritance in this important model organism.
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Despite the associated time, cost, and effort, experimental fan research remains necessary to validate computational models and physically develop new technologies. The need for a new fan research facility that would provide high quality experimental fan research at engine-representative speeds using detailed flow measurements was identified by the Office of Naval Research (ONR). The facility would be used to develop stall margin enhancement techniques, namely casing treatments to advance the field. In addition to support by ONR, Honeywell Aerospace donated a transonic fan rig and core exhaust plenum to make this project a reality.
The new research facility was designed and built around this new fan rig for investigations into casing treatments, inlet distortion, and aeromechanics research, as well as future projects that would make use of the new space. The funding package included a renovation of the build room in ZL1 and two brand new test cells constructed in previously empty space. All necessary equipment was designed, procured, and placed in the correct positions to ensure operability of the fan. The new space necessitated a mechanical checkout and commissioning process before conducting research projects.
In parallel to the development of the facility, a novel fan casing was designed to make use of rapid prototyping to experimentally test casing treatments. The fan casing assembly is made up of three metal components that remained fixed and six individual 3D printed plastic inserts that make up the flowpath surrounding the rotor. The geometry of each component was developed according to best-practices and computational structural analysis. Following commissioning of the fan test cell, the new fan casing was successfully implemented and tested over the full operating range of the fan.
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Step 1: Check the requirements. Step 2: Choose a broad field of research. Step 3: Look for books and articles. Step 4: Find a niche. Step 5: Consider the type of research. Step 6: Determine the relevance. Step 7: Make sure it's plausible. Step 8: Get your topic approved. Other interesting articles.
Craft a convincing dissertation or thesis research proposal. Write a clear, compelling introduction chapter. Undertake a thorough review of the existing research and write up a literature review. Undertake your own research. Present and interpret your findings. Draw a conclusion and discuss the implications.
Research questions must be aligned with other aspects of the thesis, dissertation, or project study proposal, such as the problem statement, research design, and analysis strategy. To summarize: Idea >Reviewing literature > Identifying the gap in theory or practice >Problem and Purpose Statements >Research question
Step 1: Choose your topic. First you have to come up with some ideas. Your thesis or dissertation topic can start out very broad. Think about the general area or field you're interested in—maybe you already have specific research interests based on classes you've taken, or maybe you had to consider your topic when applying to graduate school and writing a statement of purpose.
Secondary research plays a crucial role in dissertation writing, providing a foundation for your primary research. By leveraging existing data, you can gain valuable insights, identify research gaps, and enhance the credibility of your study. Unlike primary research, which involves collecting original data directly through experiments, surveys ...
The research process for a dissertation or project is substantial and takes time. You will need to think about what you have to find out in order to answer your research question, and where and when you can find this information. As you gather your research, keep returning to your research question to check what you are doing is relevant. ...
A dissertation is a long-form piece of academic writing based on original research conducted by you. It is usually submitted as the final step in order to finish a PhD program. Your dissertation is probably the longest piece of writing you've ever completed. It requires solid research, writing, and analysis skills, and it can be intimidating ...
A dissertation is a substantial research project that contributes to your field of study. Graduate students write a dissertation to earn their doctorate. The format and content of a dissertation vary widely depending on the academic discipline. Doctoral candidates work closely with their faculty advisor to complete and defend the dissertation ...
Make sure you can ask the critical what, who, and how questions of your research before you put pen to paper. Your research proposal should include (at least) 5 essential components : Title - provides the first taste of your research, in broad terms. Introduction - explains what you'll be researching in more detail.
So, you're finally nearing the end of your degree and it's now time to find a suitable topic for your dissertation or thesis. Or perhaps you're just starting out on your PhD research proposal and need to find a suitable area of research for your application proposal.. In this post, we'll provide a straightforward 6-step process that you can follow to ensure you arrive at a high-quality ...
Definition of Dissertation and Thesis. The dissertation or thesis is a scholarly treatise that substantiates a specific point of view as a result of original research that is conducted by students during their graduate study. At Cornell, the thesis is a requirement for the receipt of the M.A. and M.S. degrees and some professional master's ...
Revised on 10 October 2022. Your research methodology discusses and explains the data collection and analysis methods you used in your research. A key part of your thesis, dissertation, or research paper, the methodology chapter explains what you did and how you did it, allowing readers to evaluate the reliability and validity of your research.
A dissertation is a lengthy research paper written as a requirement to earn an academic degree. Typically, students must write a dissertation toward the end of their program to prove their knowledge and contribute new research to their field. Whether a student earns the degree depends on the quality of their paper and how it is presented.
The format of a dissertation may vary depending on the institution and field of study, but generally, it follows a similar structure: Title Page: This includes the title of the dissertation, the author's name, and the date of submission. Abstract: A brief summary of the dissertation's purpose, methods, and findings.
Revised on 5 May 2022. A dissertation is a large research project undertaken at the end of a degree. It involves in-depth consideration of a problem or question chosen by the student. It is usually the largest (and final) piece of written work produced during a degree. The length and structure of a dissertation vary widely depending on the ...
How to Write a Thesis Statement (Indiana University) Identify possible sources of information in many types and formats. Georgetown University Library's Research & Course Guides; Judge the scope of the project. Reevaluate the research question based on the nature and extent of information available and the parameters of the research project.
Your research methodology discusses and explains the data collection and analysis methods you used in your research. A key part of your thesis, dissertation, or research paper, the methodology chapter explains what you did and how you did it, allowing readers to evaluate the reliability and validity of your research and your dissertation topic.
Table of contents. Step 1: Coming up with an idea. Step 2: Presenting your idea in the introduction. Step 3: Exploring related research in the literature review. Step 4: Describing your methodology. Step 5: Outlining the potential implications of your research. Step 6: Creating a reference list or bibliography.
Step 1: Decide on the type of data. Step 2: Decide on primary research methodology. Steps 3 - 8 if you have chosen a qualitative method. Steps 3 - 8 if you have chosen a quantitative method. Steps 3 - 8 if you have chosen a mixed method. Other steps you need to consider. Summary.
The Dissertation Guidebook is one of the essential navigation tools Walden provides to its doctoral candidates. A vital portion of the document details the 15 required steps that take a dissertation from start to finish. Read along with Walden students to learn more about that process: Premise. The dissertation premise is a short document that ...
abstract, appendices, conclusion, discussion, essay title, introduction, literature review, method, references, results, structure. In this post, we look at the structural elements of a typical dissertation. Your department may wish you to include additional sections but the following covers all core elements you will need to work on when ...
Abstract or executive summary. The dissertation abstract (or executive summary for some degrees) serves to provide the first-time reader (and marker or moderator) with a big-picture view of your research project. It should give them an understanding of the key insights and findings from the research, without them needing to read the rest of the report - in other words, it should be able to ...
This page explains how to write references for works from academic research databases and how to provide database information in references when it is necessary to do so. ... dissertations and theses published in ProQuest Dissertations and Theses Global; Risto, A. (2014).
DISSERTATION RESEARCH: A trait-based approach to characterize microbial community composition. NSF Org: DEB Division Of Environmental Biology: Recipient: ... The investigators will present their research to local communities through the Discovery Cube Science Center of Orange County, a non-profit organization with a goal to inspire citizens ...
When starting your thesis or dissertation process, one of the first requirements is a research proposal or a prospectus. It describes what or who you want to examine, delving into why, when, where, and how you will do so, stemming from your research question and a relevant topic. The proposal or prospectus stage is crucial for the development ...
PhD candidate Alexandra Peña Hernández successfully defended her thesis: "Integrated strategies for improved management of cercospora leaf spot on sugarbeet," working with lead advisor Jaime Wilbur and the MSU/USDA Sugarbeet and Bean Research Unit.. Faculty, students and staff packed the room while Alex described her process of science and discovery—including many hours driving ...
Before I began my thesis research, a well-established dogma in the field was that parental histones containing PTMs are symmetrically distributed to daughter DNA strands during DNA replication. These modified histones serve as templates for PTM duplication, thereby restoring the original chromatin states on both daughter strands.
The research focuses on the survivors' embodied experiences and their responses to gender-based domestic violence (GBDV) within this pluralistic legal context. The study is set in Kolkata, West Bengal, a region with a significant political shift since 2011, where caste and religion have become increasingly prominent identity markers.
Despite the associated time, cost, and effort, experimental fan research remains necessary to validate computational models and physically develop new technologies. The need for a new fan research facility that would provide high quality experimental fan research at engine-representative speeds using detailed flow measurements was identified by the Office of Naval Research (ONR). The facility ...